The root-knot nematode, Meloidogyne hapla, is an increasing problem in carrot cultivation in Sweden and Europe. Soil analysis is extremely important to plan sustainable crop rotations, reduce nematode multiplication in soil and eventually minimize yield loss. Traditional soil analyses are time consuming and have low capacity. The overall goal of this project is to provide growers with a cheap and reliable DNA method based on the Loop-mediated Isothermal Amplification (LAMP). The aim is to validate the practical application of LAMP for soil analysis. DNA will be extracted from two soil types to which certain number of second-stage juveniles are added. Two DNA extraction methods will be compared, a simple manual method and a commercial DNA kit. DNA will be extracted from soil samples, collected from field trials in 2017, as well as new samples from commercial fields in Skåne and Gotland. Samples will be analyzed by Seinhorsts elutriation method and by real-time LAMP.