The Swedish dairy industry has made significant improvements in animal welfare, feeding, disease control and herd management. However, diagnosis and prevention of a very common metabolic disorder, subacute ruminal acidosis (SARA), remains a challenge. Even in well-managed dairy herds, 11 to 26% of SARA cases are suspected in cows in early to mid-lactation period, resulting in significant direct and indirect economic losses, and leading to suffering in domesticated cows. SARA lacks clear symptoms, and is difficult to diagnose with existing methods. We propose to fill this gap by identifying metabolites in bio-fluids associated with the onset and progression of SARA using state-of-the-art metabolomics techniques and developing a novel paper-based test for early detection. Our goal is to provide a novel detection method for SARA, support cattle welfare, make the dairy production more sustainable and efficient and promote climate-friendly production through optimal feed conversion.