The role of liming in the crop rotation remains to be thoroughly investigated. Within two recent SLF projects, yield in sugar beet, barley and oil seed rape has been measured in the first rotation. In 2018, beets will be grown for a second time which gives us unique possibilities with the aim to study the long-term effects of liming on diseases, plant nutrition, soil structure and effects on phosphorus which has not been done before. The objectives of the project are to measure yield in beets in rotation 2 and in winter-wheat, barley and oil seed rape in rotation 1 in 13 GPS positioned trials. We will measure total amounts of P, phosphate-P etc. in drainage water from lysimeters and relate this to long term aggregate stability. Diseases are evaluated in the field as well as in bioassays. Effects of lime on free living nematodes are evaluated. Long term effects on plant nutrition in soil and in plants will also be studied.