The ability of milk to coagulate is crucial for cheese production. We have previously shown high occurrence of non-coagulating milk from Swedish Red Dairy cattle and identified milk proteins as potential markers for this undesirable trait. In this project we will increase the understanding of the functionality of milk proteins by knowing the exact amino acid sequences and variations of casein variants. This information is not yet available due to imperfections in analytical methods. Thus, the outcome of the project will provide a unique position to contribute to a common worldwide understanding of the functionality of milk proteins and the occurrence of non-coagulating milk. This is important for industrial applications in the dairy and breeding sectors to prevent the occurrence of non-coagulating milk, but also for selection of milk for specific use and health benefits. This secures increased growth, profitable and sustainable production for Swedish milk producers and dairy industry.