Cereal cyst nematodes (CNN) are considered as major pests in cereals causing considerable yield losses, especially in monoculture systems. The status of CCNs in Sweden is not fully elucidated, but recent surveys conducted in Västra Götaland county showed wide occurrence, specially of Heterodera avenae. Precise identification of CCNs is necessary to develop effective integrated pest management. Susceptible cultivars grown frequently, as of today, may increase spread of CCNs, a scenario that we would like to avoid. The aim is to close the knowledge gap by investigating the presence of CCNs in cereal districts, assess susceptibility of spring and winter wheat, oats, barley, and rye cultivars to H. avenae and H. filipjevi at two densities in bioassays, develop a real-time PCR (qPCR) for H. hordecalis and provide guidelines to reduce yield loss. Soil samples, approx. 200, will be collected and analysed by published qPCR (H. avenae and H. filipjevi) and the new method for H. hordecalis.