Wheat bran is the largest by-product of wheat flour production with approximately 300,000 tonnes in Sweden. Bran is mainly used in animal feed, as human digestion is not fully able to absorb it. Wheat bran contains valuable biomolecules, such as dietary fiber (arabinoxylans, AX) and phenolic compounds (ferulic acid). Further processing of wheat bran therefore shows great potential for the production of food ingredients with prebiotic properties for improved health and well-being. In the proposed project we will scale up a process for isolating bioactive high molecular weight feruloylated F-AX and oligosaccharides using only subcritical water and enzymes. We will validate the prebiotic and antioxidative effects of the F-AX fractions extracted from wheat bran, as well as their effect to extend the shelf life of bread. This will increase the profitability for wheat bran, and will offer new bread products with increased durability that possibly contribute to better health and well-being.